Chapter 16
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Previous chapters answered one question at a time: Does training affect strength? Does group predict aerobic capacity?
These are incomplete. In practice, researchers ask:
Did a 12-week training program produce strength gains, and did those gains differ by sex or across time?
| Control | Training | |
|---|---|---|
| Female | Female-Control | Female-Training |
| Male | Male-Control | Male-Training |
A 3 × 3 design has 9 cells. Adding a third 2-level factor creates 2 × 3 × 2 = 12 cells. Most applied research uses two-factor designs.
In a 2(Group: Control/Training) × 2(Sex: Female/Male) factorial design, what does the cell mean for "Female-Training" specifically represent?
One model, controlled error rate, smaller error term, and — crucially — the ability to detect and estimate interactions.
Whether a training program benefits males and females differently is a question that lives only inside a factorial design. It cannot be answered any other way.
A researcher runs two separate one-way ANOVAs — one for Group, one for Sex — instead of a single factorial ANOVA. Which problem is impossible to fix within that approach?
A main effect is the overall effect of one factor, averaging — collapsing — across all levels of the other factor(s).
Main effects tell the simple, unconditional story — and they are perfectly interpretable when no interaction is present.
The main effect averages two different patterns into one number that accurately describes neither. It is technically correct but substantively misleading.
To get the main effect of Group, average across Sex ↓
| Control | Training | |
|---|---|---|
| Female | 77.54 | 87.18 |
| Male | 76.22 | 83.64 |
| ⬇ average across Sex | ||
| Marginal M | 77.14 | 85.06 |
An interaction occurs when the effect of one factor depends on the level of another factor.
Both groups change in the same direction, but the magnitude differs. Lines diverge — do not cross. Example: training adds 12 kg for males, 4 kg for females — both improve.
The direction reverses across levels — training helps one group but impairs another. Lines cross. Rare but scientifically dramatic.
Always interpret the interaction before the main effects. A significant interaction means the main effects are incomplete summaries.
| Type | Pattern | Lines |
|---|---|---|
| Quantitative | Same direction, different magnitude | Diverge |
| Qualitative | Direction reverses | Cross |
| None | Identical effect across groups | Parallel |
Quantitative interactions are common in movement science; qualitative interactions are rare but most compelling.
A 2×2 factorial ANOVA shows a significant main effect of Group (p = .003) and a non-significant interaction (p = .54). What is the correct next step?
Parallel → interpret main effects directly
Diverge — same direction, different magnitude
Cross — direction reverses between groups
Are the lines parallel? Yes → no interaction, interpret main effects. No → interaction present, run simple effects before interpreting anything else.
The plot shows training improved both groups, but Females gained far more than Males. What type of interaction is this?
Every participant belongs to exactly one cell. Total variance splits into four sources:
All three effects share the same within-cell error term:
| Source | df | Denominator |
|---|---|---|
| Factor A | a − 1 | MSerror |
| Factor B | b − 1 | MSerror |
| A × B Interaction | (a−1)(b−1) | MSerror |
| Error (within-cell) | N − ab | — |
a = levels of A · b = levels of B · N = total sample size · ab = number of cells
Focus on what each source tests and which error term it uses. You must understand this to interpret the output correctly.
Did a 12-week training program improve post-test strength, and did this effect differ between male and female participants? N = 60
| Sex | Group | n | M (kg) | SD |
|---|---|---|---|---|
| Female | Control | 21 | 77.54 | 14.70 |
| Female | Training | 12 | 87.18 | 8.21 |
| Male | Control | 9 | 76.22 | 12.92 |
| Male | Training | 18 | 83.64 | 14.72 |
| Group | n | M (kg) | SD |
|---|---|---|---|
| Control (total) | 30 | 77.14 | 13.98 |
| Training (total) | 30 | 85.06 | 12.48 |
Unbalanced design (unequal cell n) → SPSS uses Type III SS automatically.
Look at the cell means visually first. Do the female and male training gains look similar in magnitude? That will tell you what the interaction test should show.
| Source | SS | df | MS | F | p | η²p |
|---|---|---|---|---|---|---|
| Sex | 0.22 | 1 | 0.22 | 0.00 | .974 | .000 |
| Group | 939.31 | 1 | 939.31 | 5.22 | .026 | .085 |
| Sex × Group | 101.14 | 1 | 101.14 | 0.56 | .457 | .010 |
| Error | 10,084.70 | 56 | 180.08 | — | ||
Sex × Group: F(1,56) = 0.56, p = .457, η²p = .010 — not significant, negligible effect. The training benefit did not differ between males and females. ✓ Main effects can be interpreted.
Group: F(1,56) = 5.22, p = .026, η²p = .085 — significant, medium effect. Training (85.06 kg) outperformed Control (77.14 kg).
Sex: F(1,56) = 0.00, p = .974 — not significant. Males and females had similar post-test strength.
The same training prescription was equally effective for both sexes. No sex-specific program modifications are indicated. Null interaction results are not failures.
The mixed factorial ANOVA (also: split-plot design) combines:
Do groups differ in their trajectory of change over time? Neither a between-subjects ANOVA nor a repeated-measures ANOVA alone can answer this.
The Group × Time interaction is the scientific heart of most longitudinal intervention studies in movement science.
Between-subjects variation is large — people differ enormously in baseline strength.
Within-subjects variation is much smaller — it captures only how inconsistently each person responds across time, stripped of their individual baseline.
Within-subjects F-ratios are not comparable in magnitude to between-subjects F-ratios. Use η²p to compare effect sizes across sources.
A 2(Group: Control/Training) × 2(Sex: Female/Male) ANOVA yields: main effect of Group p = .001, main effect of Sex p = .04, interaction p = .71. Which statement is best supported by these results?
| Effect | Type | Error Term | Why? |
|---|---|---|---|
| Group | Between-subjects | MSSubjects/Group | Captures stable individual differences in baseline strength |
| Time | Within-subjects | MSTime×Subj/Group | Individual inconsistency across time only, after removing baselines |
| Group × Time | Within-subjects | MSTime×Subj/Group | Same within-subjects error as Time |
Using the within-subjects error for the Group effect produces an artificially large F, because MSwithin is far smaller than MSSubjects/Group. SPSS prevents this automatically in Repeated Measures GLM.
A researcher is puzzled: "Why does SPSS report a different error term for the Group effect than for the Time effect in my mixed ANOVA?" Which explanation is correct?
SSSubj/Group = between-subjects error → denominator for FGroup
SSTime×Subj/Group = within-subjects error → denominator for FTime and FGroup×Time
The between-subjects error reflects all stable individual differences in absolute strength — a large, noisy term.
The within-subjects error captures only how inconsistently each person responds across time, after removing individual baselines — typically much smaller.
Time and Group×Time effects are tested against the small within-subjects error → much higher statistical power than the Group effect, which uses the large between-subjects error.
Sphericity requires that the variances of all pairwise differences among within-subjects levels be approximately equal. In the mixed design, this applies to:
| Mauchly's Result | Action |
|---|---|
| p > .05 | Sphericity Assumed — use those rows for Time and Group×Time |
| p < .05, εGG < .75 | Greenhouse-Geisser correction — apply to Time and Group×Time |
| p < .05, εGG ≥ .75 | Huynh-Feldt correction — apply to Time and Group×Time |
Report Mauchly's W, df, and p. If a correction was applied, report the epsilon (ε) value and state which correction was used — even when sphericity is not violated.
Did strength change over a 12-week training program, and did the trajectory of change differ between the training and control groups? n = 30 per group; outcome: strength (kg)
| Group | Pre | Mid | Post |
|---|---|---|---|
| Control | 76.34 (13.70) | 76.85 (13.87) | 77.14 (13.98) |
| Training | 79.67 (12.26) | 81.69 (12.26) | 85.06 (12.48) |
Before reading ANOVA output: inspect the pattern. Control is essentially flat. Training climbs steadily. Expect a significant Group×Time interaction.
Mauchly's W(2) = .93, p = .059
Not violated (p > .05) → use Sphericity Assumed rows for Time and Group×Time
If you read the Sphericity Assumed F-values when sphericity is violated, your p-values will be too small and you'll over-reject H₀. Always check Mauchly's before reading Time and interaction results.
Mauchly's test of sphericity is significant (W = .68, p = .02). What is the correct next step?
| Source | df | MS | F | p | η²p | ω²p |
|---|---|---|---|---|---|---|
| Group | 1 | 1,294.44 | 2.52 | .117 | .042 | .008 |
| Subjects/Group (error B) | 58 | 513.32 | — | |||
| Time | 2 | 145.24 | 108.55 | <.001 | .652 | .544 |
| Group × Time | 2 | 81.63 | 61.00 | <.001 | .513 | .400 |
| Time × Subj/Group (error W) | 116 | 1.34 | — | |||
Group × Time: F(2,116) = 61.00, p < .001, η²p = .513, ω²p = .400 — a large effect. The groups followed different strength trajectories. → Run simple effects; interpret main effects in context.
Groups start at similar levels and diverge over time. Their time-averaged means aren't dramatically different — so the Group main effect (p = .117) misses the pattern the interaction reveals. The interaction is more sensitive to diverging trajectories.
Significant interaction → decompose with simple effects analysis before drawing conclusions about Time or Group.
Simple effects test the effect of one factor separately at each level of the other factor. Run only after a significant omnibus interaction.
Only after a statistically significant interaction. Running them after a non-significant interaction capitalizes on chance and inflates Type I error — the omnibus test is your gate.
General Linear Model → Repeated Measures → Options → Compare main effects → Bonferroni. Or: split file by Group, run separate one-way repeated-measures ANOVAs.
The interaction plot tells you where the interaction lives. Run only theoretically motivated simple effects — not all possible combinations.
Reported automatically by SPSS
The "partial" means each effect is sized relative to its own error term, not total variance. This correctly isolates each effect.
| Effect | Error in denominator |
|---|---|
| Group | SSSubjects/Group (between) |
| Time | SSTime×Subj/Group (within) |
| Group × Time | SSTime×Subj/Group (within) |
Less biased — recommended for small-to-moderate samples
Small ≈ .01 | Medium ≈ .06 | Large ≥ .14
Well-controlled lab training studies routinely produce η²p > .50 for within-subjects effects. That's not unusual — it reflects the favorable signal-to-noise ratio of within-subject designs.
η²p AND ω²p for Group, Time, and the interaction — including non-significant effects. Readers need all values to evaluate power.
A Group × Time interaction is significant. Simple effects show the Group effect at Time 1 is F(1, 46) = 0.23, p = .63. What does this finding mean?
The Group × Time interaction yields η²p = .42, p < .001. A colleague concludes: "The training program caused 42% of all outcome variance." What is wrong with this statement?
G*Power requires Cohen's f — not η²p — as its effect size input for factorial ANOVA power analyses.
| Effect | η²p | f | Interpretation |
|---|---|---|---|
| Group × Time | .513 | 1.03 | Very large |
| Time | .652 | 1.37 | Very large |
| Group | .042 | 0.21 | Small-medium |
The interaction (f = 1.03) and Time (f = 1.37) have enormous effect sizes because they're tested against the tiny within-subjects error. Group (f = 0.21) uses the noisy between-subjects error — it requires a much larger N to achieve equivalent power.
Use: F-test → ANOVA: Repeated Measures, between-within interaction. Key extra input: expected correlation among repeated measures. In training studies with strength, pre-mid-post correlations are typically r = .96–.98, explaining the very high power of within-subjects effects.
Required order — always follow this sequence:
Report η²p and ω²p for every source. A non-significant Group effect with η²p = .04 tells a different story than one with η²p = .30 that a study was underpowered to detect.
"A 2 (Group: control, training) × 3 (Time: pre, mid, post) mixed ANOVA was conducted with strength (kg) as the dependent variable. Mauchly's test indicated that the sphericity assumption was not violated, W(2) = .93, p = .059.
The Group × Time interaction was statistically significant, F(2, 116) = 61.00, p < .001, η²p = .51, ω²p = .40, indicating that the two groups followed different strength trajectories over the 12-week program. Simple effects analysis revealed a significant effect of Time within the training group, with progressive gains from pre-test (M = 79.67 kg, SD = 12.26) to post-test (M = 85.06 kg, SD = 12.48). The effect of Time was not significant within the control group, which remained essentially unchanged (76.34, 76.85, 77.14 kg).
The main effect of Time was significant, F(2, 116) = 108.55, p < .001, η²p = .65, ω²p = .54. The main effect of Group was not significant, F(1, 58) = 2.52, p = .117, η²p = .04, ω²p = .008."
Which of the following correctly reports a mixed ANOVA interaction effect in APA format?
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